ferulic acid protects pc12 neurons against hypoxia by inhibiting the p-mapks and cox-2 pathways

نویسندگان

wen-chieh lin department of optometry, yuanpei university, hsinchu, taiwan

yu-fen peng department of physical education office, yuanpei university, hsinchu, taiwan

chien-wei hou department of biotechnology, yuanpei university, hsinchu, taiwan

چکیده

objective(s):hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. here, the protective effects of ferulic acid (fa) on hypoxia-induced neurotoxicity in pc12 cells were evaluated. materials and methods:we investigated the effect of fa on pc12 cells subjected to hypoxia stress, in vitro. results:fa increased cell viability, prevented membrane damage (ldh release), scavenged free radicals, increased superoxide dismutase (sod) activity, and attenuated the elevation of intracellular free ca2+, lipid peroxidation, apoptosis (evaluated by tunel staining) and pge2 production in hypoxia-stressed pc12 cells. mapks were activated during hypoxia. fa reduced p-p38 mapk, caspase-3, and cox-2 activation which correlated well with diminished ldh release in pc12 cells under hypoxia. furthermore, fa reduced lipid peroxidation in pc12 cells subjected to hypoxia. conclusion:taken together, these results indicate that fa antioxidant effects could partly be involved in inhibition of p38 mapk pathway and apoptosis through scavenging ros in hypoxia-stressed pc12 cells.

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Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways

Objective(s):Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. Here, the protective effects of ferulic acid (FA) on hypoxia-induced neurotoxicity in PC12 cells were evaluated. Materials and Methods:We investigated the effect of FA on PC12 cells subjected to hypoxia stress, in vitro. Results:FA increased cell viability, prevented membrane damage (LDH r...

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Ferulic acid protects PC12 neurons against hypoxia by inhibiting the p-MAPKs and COX-2 pathways

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عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۵، صفحات ۴۷۸-۴۸۴

کلمات کلیدی
[ ' c a s p a s e ' , 3 , ' f e r u l i c a c i d ' , ' h y p o x i a ' , ' m a p k s ' , ' r o s ' ]

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